Downloading Data

1 Download & save files in directory

You can download the files for your project from QIITA.

• Go to the Study Dropdown, and select “View Study”
• Click on the study title you are interested in
• Click on “Download All Data”, including metadata files.

Alternatively, these files can be found on the BioCompute CIFS share at Boise State University.

You will get three files from UCSD, including R1, R2, I1, and map files. You will rename the files as follows: - R1 -> forard.fastq.gz - R2 -> reverse.fastq.gz - I1 -> barcodes.fastq.gz

With the mapping file, ensure that your first two columns are titled “sample_name” and “BarcodeSequence”.

2 Move all files to your cluster using secure copy (scp)

You will move all files to the cluster using the command scp. Ensure that you are moving these to a scratch area, where there is enough space to hold large files:

cd local_folder_containing_files
scp * sgalla@borah-login.boisestate.edu:~/scratch/Pronghorn_16S/

3 Move all files to your cluster using Globus

Alternatively, at Boise State University you can use Globus to move files from the BioCompute CIFS share to your scratch folder.

• Sign into Globus with your Boise State University Credentials.
• In the left search bar, do a ‘collection search’ for Globus-VM (Boise State University).
• Navigate to the BioCompute CIFS Share and select the folder you want to move.
• In the right search bar, do a ‘collection search’ for BORAH-DTN.
• Navigate to the SCRATCH folder, find your scratch, and select the folder you want to move data towards.
• Press ‘GO’